Defined systems for epithelial cell culture and use thereof
US6692961B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 26, 2000 |
| Grant date | Feb 17, 2004 |
| Priority date | — |
| Expiry date | Nov 15, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/113
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods of cultivating animal epithelial cells in vitro using these cell culture media formulations, kits comprising the media, cell culture compositions comprising the culture media and an animal epithelial cell, and compositions that may be used as replacements for organ or gland extracts in animal cell culture media.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.