Protein/(poly)peptide libraries

US6696248B1 · kind B1 · utility

Assignee

• MORPHOSYS AG · DE

Inventors

• Achim Knappik · Gräfelfing, DE
• Peter Pack · Unterföhring, DE
• Liming Ge · Munich, DE
• Simon Moroney · Hersbruck, DE
• Andreas Plückthun · Zürich, CH

Key dates

• Filing date: Jan 24, 2000
• Grant date: Feb 24, 2004
• Expiry date: Jan 24, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to synthetic DNA sequences which encode one or more collections of homologous proteins/(poly)peptides, and methods for generating and applying libraries of these DNA sequences. In particular, the invention relates to the preparation of a library of human-derived antibody genes by the use of synthetic consensus sequences which cover the structural repertoire of antibodies encoded in the human genome. Furthermore, the invention relates to the use of a single consensus antibody gene as a universal framework for highly diverse antibody libraries.