Method, test media and chromogenic compounds for identifying and differentiating general coliforms and escherichia coli bacteria
US6699685B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 27, 1995 |
| Grant date | Mar 2, 2004 |
| Priority date | — |
| Expiry date | Feb 27, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A chromogenic &bgr;-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by &bgr;-galactosidase and a chromogenic &bgr;-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by &bgr;-glucuronidase are combined in a test medium for quantitatively identifying and differentiating general coliforms and E. coli. The &bgr;-galactosidase substrate 5-bromo-4-chloro-3-indolyl-&bgr;-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by &bgr;-galactosidase may be used with one of the novel compounds 6-chloroindolyl-&bgr;-D-glucuronide, 4,6-dichloroindolyl-&bgr;-D-glucuronide, 6,7-dichloroindolyl-&bgr;-D-glucuronide, and 4,6,7-trichloroindolyl-&bgr;-D-glucuronide, which produce mauve or magenta precipitates when reacted upon by &bgr;-glucuronidase. The &bgr;-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-&bgr;-D-glucuronide, which produces an indigo blue precipitate when reacted upon by &bgr;-glucuronidase may be used with one of the novel compounds 6-chloroindolyl-&bgr;-D-galactoside, 4,6-dichloroindolyl-&bgr;-D-galactoside, 6,7-dichloroindolyl-&bgr;-D-galactoside, a…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.