Modified nuclear glucocorticoid receptor, fusion protein, and DNA fragments coding for said receptor and said fusion protein

US6699686B1 · kind B1 · utility

Assignee

• Association pour le Development de la Recherche en Genetique Moleculaire (ADEREGEM) · FR

Inventors

• Jacques Bertrand Brocard · Strasbourg, FR
• Pierre Chambon · Blaesheim, FR
• Hinrich Gronemeyer · Oberkirch, DE
• Daniel Metzger · Belleville, US
• Jean-Claude Nicolas · Montpellier, FR
• Sylvie Roux · Montpellier, FR

Key dates

• Filing date: Aug 20, 1998
• Grant date: Mar 2, 2004
• Expiry date: Aug 20, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A DNA fragment coding for a modified nuclear glucocorticoid receptor, particularly one mutated in the region coding for the ligand binding domain, so that receptor activity is more strongly inducible by a synthetic glucocorticoid ligand than by a natural glucocorticoid ligand, is disclosed. A recombination system inducible in mammals by means of a fusion protein produced between a recombinase and the binding domain of the ligand derived from the modified glucocorticoid receptor of which the activity is more strongly inducible by synthetic glucocorticoids than by natural glucocorticoids, is also disclosed.