Electrophoretic separation of nucleic acids from proteins at low ph
US6699986B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 9, 1999 |
| Grant date | Mar 2, 2004 |
| Priority date | — |
| Expiry date | Jul 9, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for separating nucleic acids from other cellular debris, especially substances that carry a net positive charge at low pH, by electrophoresis under acid conditions. In the purification method of the present invention, nucleic acids are separated from proteins found in the same biological sample by applying the sample to an electrophesis gel and subjecting the sample to electrophoresis under acid conditions to separate the nucleic acids from the proteins. The optimum pH may differ for different sample types but can be readily determined by those skilled in the art. Preferably, the separation is performed at a pH of about 2 to about 4. More preferably, electrophoresis is carried out at a pH of 2.5
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.