General purpose structure of an analytical element and its application for analyte determination

US6703196B1 · kind B1 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Jürgen Klepp · Karlsruhe, DE
• Gerhard Hiller · Mannheim, DE
• Alfons Nichtl · Hohenpeißenberg, DE
• Ulrike Fischer · Penzberg, DE
• Martina Hösch · Mannheim, DE

Key dates

• Filing date: Dec 18, 1998
• Grant date: Mar 9, 2004
• Expiry date: Dec 18, 2018

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns an analytical element for the determination of an analyte containing in or on material which enables liquid transport between zones, a sample application zone and a detection zone located downstream thereof, wherein the detection zone contains a partner 1 of a specific binding pair 1 immobilized in such a manner that it is able to bind to partner 2 of the specific binding pair 1 which is not the analyte when it contacts it, whereina labelled partner 1 of a specific binding pair 2 is present upstream of the detection zone impregnated on a material such that it can be detached by liquid and is able to bind to partner 2 of the specific binding pair 2 which is not the analyte when this contacts it as well as a method for the determination of an analyte using this analytical element.