Detection of nucleic acid sequences by invader-directed cleavage
US6706471B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 14, 1999 |
| Grant date | Mar 16, 2004 |
| Priority date | — |
| Expiry date | Jun 14, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5′ nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.