Method for controlling the release of granules
US6706683B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 29, 2001 |
| Grant date | Mar 16, 2004 |
| Priority date | — |
| Expiry date | Mar 29, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5094
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of controlling granule secretion comprising performing a treatment to increase or decrease an active form of calgranulin on a cell line having capability of secreting granules, thereby increasing or decreasing granule secretion from the cell line.A method of detecting a substance which inhibits or activates the granule secretion reaction by applying this controlling method. An active form of calgranulin is increased by making the cells permeabilized cells and adding a calgranulin and soluble calcium. Decrease of an active form of calgranulin is carried out by introducing a calgranulin antibody into cells.The method is useful for screening a substance which inhibits or activates granule secretion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.