Reconstitution of purified membrane proteins into preformed liposomes
US6706861B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 2001 |
| Grant date | Mar 16, 2004 |
| Priority date | — |
| Expiry date | Mar 22, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/829
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present application relates to a method of making liposomes having membrane proteins incorporated therein, the method comprising: providing the membrane protein in solution; providing a solution of preformed liposomes; and incubating the mixture. Prior to the step of providing a solution of preformed liposomes, the liposomes are formed by combining a mixture of phospholipids with a solution of at least one type of unsaturated fatty acid. The methods of the present invention further relate to the method of making a reagent comprising tissue factor reconstituted into preformed liposomes. The method of the present invention for making a tissue factor reagent comprises: providing tissue factor in solution; providing a solution of preformed liposomes comprising a mixture of phospholipids and at least one type of unsaturated fatty acid; and incubating the mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.