Diagnostic compositions, elements, methods and test kits for amplification and detection of human CMV DNA using primers having matched melting temperatures
US6709813B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 14, 1993 |
| Grant date | Mar 23, 2004 |
| Priority date | — |
| Expiry date | May 14, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An aqueous composition containing primers for opposing strands of human cytomegaloviral DNA and a second target DNA can be used in polymerase chain reaction to provide simultaneously rapid and efficient amplification and detection of those nucleic acids. The primers for each target DNA differ in length by no more than 5 nucleotides and have a Tm within the range of from about 65 to about 74° C., while the Tm's are within about 5° C. of each other. Such compositions are useful in diagnostic test kits and methods for amplification and detection of hCMV DNA and other target DNA's using multiple capture probes, in “multiplexing”. All of the capture probes have Tm's which are greater than 50° C. and are within 15° C. of each other.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.