Fluorescent energy transfer mediated chemical activation (fetma) for the elucidation of the three-dimensional structure of biomacromolecules

US6713256B1 · kind B1 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Daniel Hoffmann · Bonn, DE
• Ralf Zimmer · Bonn, DE

Key dates

• Filing date: Aug 14, 2000
• Grant date: Mar 30, 2004
• Expiry date: Aug 14, 2020

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/13
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a method for the selected chemical activation of photo-activatable cross-linker molecules around ligand binding pockets and fluorescent groups in macromolecules, especially biological macromolecules, by using fluorescent ligands of the macromolecule and by selecting photo-activatable cross-linker molecules having specific activation energies so that a radiationless energy transfer (Förster transfer) from the fluorescent ligands to the cross-linker molecules, which become activated thereby, takes place.