Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation

US6714875B1 · kind B1 · utility

Assignee

• Neogenesis Drug Discovery, Inc. · US

Inventors

• Huw M. Nash · Lexington, US
• Seth Birnbaum · Boston, US
• Edward A. Wintner · Belmont, US
• Krishna Kalghatgi · Westborough, US
• Gerald W. Shipps, Jr. · Stoneham, US
• Satish Jindal · Milton, US

Key dates

• Filing date: Aug 11, 1999
• Grant date: Mar 30, 2004
• Expiry date: Aug 11, 2019

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/15
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a method for producing a mass-coded combinatorial library comprising a set of compounds having the general formula X(Y)n, where X is a scaffold, each Y is, independently, a peripheral moiety, and n is an integer greater than 1. The method comprises selecting a peripheral moiety precursor subset from a peripheral moiety precursor set. The subset includes a sufficient number of peripheral moiety precursors that at least about 50 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors in the subset exist. The subset of peripheral moiety precursors is selected so that at least about 90% of all possible combinations of n peripheral moieties derived from the subset have a molecular mass sum which is distinct from the molecular mass sums of all of the other combinations of n peripheral moieties. The method further comprises contacting the peripheral moiety precursor subset with a scaffold precursor which has n reactive groups. Methods of use of the mass-coded combinatorial library produced by this method for identifying a ligand to a particular biomolecule are also disclosed.