Osp-C derived peptide fragments

US6716574B2 · kind B2 · utility

Assignee

• Dako A/S · DK

Inventors

• Marianne Jartved Mathiesen · Hellerup, DK
• Michael Theisen · Struer, DK
• Arne Holm · Paseo Los Muchachos, DK
• Søren Østergaard · Copenhagen, DK

Key dates

• Filing date: Oct 10, 2001
• Grant date: Apr 6, 2004
• Expiry date: Nov 11, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A diagnostic method is disclosed which utilizes short C-terminal fragments of Borrelia burgdorferi sensu lato derived protein OspC. The 4 amino-terminal acids Pro-Lys-Pro (SEQ ID NO: 22) are shown to be essential in immune reactivity between sera from patients suffering from early borreliosis and various OspC derivatives and it is shown that in order to be effective as a diagnostic agent, 5 consecutive amino acid residues long homologue of a fragment identical to the 10 C-terminal amino acids of OspC (SEQ DI NO: 1). Also disclosed are vaccines utilizing the short peptides as well as methods for their preparation.