Mobility-Modifying Cyanine Dyes
US6716994B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 4, 2000 |
| Grant date | Apr 6, 2004 |
| Priority date | — |
| Expiry date | Jan 4, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.