Detection of clonal T-cell receptor-&ggr; gene rearrangement by PCR/temporal temperature gradient gel electrophoresis (TTGE)
US6720144B1 · kind B1 · utility
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Key dates
| Filing date | Jun 21, 2001 |
| Grant date | Apr 13, 2004 |
| Priority date | — |
| Expiry date | Jun 21, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods and compositions for detecting and analyzing clonal T-cell receptor (TCR) gene rearrangement using temporal temperature gradient gel electrophoresis (TTGE), which employs a gradual and uniform increase in the temperature of a constant denaturing gel to resolve different DNA molecules based on base pair composition. The present invention also provides methods and compositions for providing appropriate DNA migration markers for TTGE analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.