Therapeutic platelets and methods

US6723497B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Willem Wolkers · Davis, US
• John Crowe · Davis, US
• Fern Tablin · Davis, US
• Ann Oliver · Davis, US
• Naomi Walker · Davis, US
• Nelly Tsvetkova · Davis, US

Key dates

• Filing date: Apr 5, 2001
• Grant date: Apr 20, 2004
• Expiry date: Jun 9, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/108331
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A dehydrated composition is provided that includes freeze-dried platelets. The platelets are loaded with trehalose which preserves biological properties during freeze-drying and rehydration. The trehalose loading is conducted at a temperature of from greater than about 25° C. to less than about 40° C., most preferably at 37° C., with the loading solution having trehalose in an amount from about 10 mM to about 50 mM. These freeze-dried platelets are substantially shelf-stable and are rehydratable so as to have a normal response to an agonist, for example, thrombin, with virtually all of the platelets participating in clot formation within about three minutes at 37° C.