Cell-based screening methods
US6730492B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 9, 2002 |
| Grant date | May 4, 2004 |
| Priority date | — |
| Expiry date | Mar 9, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cell-based screening methods for determining kinase activity are provided. The methods utilize existing cellular pathways that are regulated by kinases. In one embodiment, various components of a ubiquitin-mediated degradation pathway are modified to create an assay that can be used to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the degradation pathway. In another embodiment, various components of a protein translocation pathway are modified to screen for a molecule that modulates the activity of a kinase of interest that otherwise does not regulate the translocation pathway.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.