Methods for generating a continuous nucleotide sequence from noncontiguous nucleotide sequences
US6730500B1 · kind B1 · utility
3Cited by
3References
17Claims
0Family size
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Key dates
| Filing date | Aug 23, 2001 |
| Grant date | May 4, 2004 |
| Priority date | — |
| Expiry date | Sep 28, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The increased use of nucleotide sequence data mining techniques has amplified the demand for efficient methods of producing recombinant proteins in host cells. New genes identified from genomic sequences may lack an identified source for obtaining an mRNA template to produce cDNA. A strategy is provided for generating nucleic acid molecules comprising a continuous amino acid-encoding sequence using a genomic template that contains non-continuous amino acid-encoding sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.