Material and method for engraftment of a composite biocompatible skin graft on the neodermis of artificial skin
US6733530B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 2, 1999 |
| Grant date | May 11, 2004 |
| Priority date | — |
| Expiry date | Aug 2, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61F2/105
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
Autologous cultured keratinocytes are grown on a biosynthetic and biocompatible substratum following pre-seeding with autologous or allogenic dermal fibroblasts. The resultant composite material may then be applied on the neodermis of artificial skin which had been previously engrafted on the patient. The composite material, and specifically Composite Biocompatible Skin Graft (CBSG) material comprises autologous keratinocytes A and allogenic or autologous dermal fibroblasts grown on an artificial skin. A method for cultivating the CBSG includes the application of dermal fibroblasts onto the substratum as a feeder layer and then the inoculation of autologous keratinocytes on the resultant structure. A method for engraftment comprises first applying an artificial skin with a protective silicone membrane on a wound area, thereby allowing vascularization; and following vascularization, removing the silicone membrane and engrafting the CBSG material onto the vascularized artificial skin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.