Cationic reagents of transfection

US6733777B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Christoph Erbacher · Haan, DE
• Martin Weber · Borgo San Lazzaro, DE

Key dates

• Filing date: May 4, 1999
• Grant date: May 11, 2004
• Expiry date: May 4, 2019

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K48/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Cationic cytofectins and liposomes comprising the same are disclosed which are especially useful for delivering exogenous compounds into cells in vitro and in vivo. The liposomes may comprise (a) a neutral lipid such as dioleoylphosphatidyl-ethanolamine (DOPE) or similar lipid-like compounds such as 1,2-dioleoyl-oxiphosphatidylethanolamine or other lipid-like structures and (b) one or more of the cationic cytofectins provided herein. The invention provides transfection kits and methods for delivery of exogenous compounds into cells.