Expression monitoring for gene function identification
US6733969B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 18, 2001 |
| Grant date | May 11, 2004 |
| Priority date | — |
| Expiry date | Apr 18, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods, compositions and apparatus for mapping the regulatory relationship among genes by massive parallel monitoring gene expression. In some embodiments, mutations in the up-stream regulatory genes are detected by monitoring the change in down-stream gene expression. Similarly, the function of a specific mutation in a up-stream gene is determined by monitoring the down-stream gene expression. In addition, regulatory function of a target gene can be determined by monitoring the expression of a large number of down-stream genes. The invention also provides specific embodiments for detecting p53 functional homozygous and heterozygous mutations and for determining the function of p53 mutations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.