Coniothyrium minitans &bgr;-(1,3) exoglucanase gene cbeg 1
US6734344B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 8, 2000 |
| Grant date | May 11, 2004 |
| Priority date | — |
| Expiry date | Dec 8, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01058
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides the nucleotide sequence of a novel &bgr;-(1,3) exoglucanase gene denoted as cbeg1 of the soil-borne fungus Coniothyrium minitans. The deduced amino acid sequence of the encoded &bgr;-(1,3) exoglucanase enzyme, denoted Cbeg1, is also provided. Encoded &bgr;-(1,3) exoglucanase Cbeg1 is specific for the substrate laminarin, in that results showed no activity with other substrates tested, such as carboxymethylcellulose, barley &bgr;-glucan, lichenan, oat spelt xylan and birchwood xylan. The pH and temperature optima for &bgr;-(1,3) exoglucanase Cbeg1 are 6.0 and 57° C., respectively. Cbeg1 contains 784 amino acids, and has a predicted isoelectric point (pI) of 6.0 and molecular weight of 83,646 Daltons. The invention further provides vectors and cells comprising a nucleic acid molecule encoding the cbeg1 gene, and methods for producing &bgr;-(1,3) exoglucanase Cbeg1. The cbeg1 gene is compatible with a eukaryotic heterologous expression system, making it particularly useful for a wide range of industrial applications, such as improvement of plant resistance to fungal phytopathogens or use in ruminant microbial transgenic strategies to improve feed digestion a…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.