High-sensitive detection of environmental pollutants
US6740521B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 11, 2002 |
| Grant date | May 25, 2004 |
| Priority date | — |
| Expiry date | Mar 11, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a bioassay system, for detection of hazardous chemical substances, natural toxic substances in the environment and unknown toxic compounds, with high sensitivity, simplicity and speed. The invention provides cells and a method of using the cells for use in the bioassay system. The cell provided by the invention contains a heat shock factor binding DNA sequence and a transcriptional regulatory sequence necessary on an occasion of stress induction as a transcriptional regulatory factor binding site. The cell also possesses a reporter gene under the control of the promoter. The reporter gene is connected, on the downstream side, to the SV40pA signal without any intervening intron.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.