Process for continuous purification and concentration of leukocytes from blood
US6743624B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 17, 2000 |
| Grant date | Jun 1, 2004 |
| Priority date | — |
| Expiry date | Nov 17, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/0087
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Process and apparatus for the continuous purification and concentration of leukocytes from blood, characterized in that said process comprises the following steps: (a) seperating plasma from the blood by filtration in order to achieve a filtered buffy coat fraction; (b) adding an aqueous solution, which is hypotonic in relation to plasma, to the buffy coat fraction from step (a), in order to achieve lysation of erythrocytes contained in the buffy coat fraction; (c) mixing the buffy coat fraction and the aqueous hypotonic solution from step (b) in a mixing device; (d) leading the mixture from step (c) through a retention vessel; (e) leading the mixture from step (d) through a centrifuge in order to seperate the leukocytes; (f) collecting the separated leukocytes from step (e).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.