Nucleic acid binding proteins
US6746838B1 · kind B1 · utility
103Cited by
35References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 29, 2000 |
| Grant date | Jun 8, 2004 |
| Priority date | — |
| Expiry date | Feb 29, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1037
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method for preparing a nucleic acid binding protein of the Cys2-His2 zinc finger class capable of binding to a nucleic acid quadruplet in a target nucleic acid sequence, wherein binding to base 4 of the quadruplet by an alpha-helical zinc finger nucleic acid binding motif in the protein is determined as follows: if base 4 in the quadruplet is A, then position +6 in the alpha-helix is Glu, Asn or Val; if base 4 in the quadruplet is C, then position +6 in the alpha-helix is Ser, Thr, Val, Ala, Glu or Asn.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.