Immunoassays involving surface enhanced Raman scattering
US6750065B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 20, 2000 |
| Grant date | Jun 15, 2004 |
| Priority date | — |
| Expiry date | Nov 20, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Immunoassay displacement methods for detecting (preferably identifying) the presence or amount of a target analyte (e.g. drug) in a sample comprising the steps of: (a) exposing the sample to a complex comprising an immobilized antibody capable of specifically binding the analyte, said antibody being specifically bound to a displacement agent, wherein said agent comprises: (I) an analyte analog capable of specifically binding the antibody, (ii) a surface enhanced resonance raman scattering (SERRS) or a surface enhanced raman scattering (SERS) active label, (iii) a SERRS or SERS surface-seeking group (e.g. benztriazole), whereby any analyte present in the sample causes the displacement of agent from the antibody; (b) exposing any displaced agent to a SERRS or SERS surface; and, (c) detecting any displaced agent associated with said surface using SERRS or SERS. In preferred forms of the invention several target analytes are detected from a single source or sample simultaneously. Also provided are agents per se e.g. for use in drug or explosive detection. These may include chromophores and linking groups.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.