Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
US6753145B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 5, 2001 |
| Grant date | Jun 22, 2004 |
| Priority date | — |
| Expiry date | Jul 5, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving theintactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH within a range of pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from about 0.01 M to about 2.0 M, and optionally relatively lower concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with the microarray in the buffer composition at a temperature between about 55° C. and 70° C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.