Methods for making proteins containing free cysteine residues
US6753165B1 · kind B1 · utility
82Cited by
2References
54Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 6, 2001 |
| Grant date | Jun 22, 2004 |
| Priority date | — |
| Expiry date | Sep 6, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/72
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.