Metastasis models using green fluorescent protein (GFP) as a marker
US6759038B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 29, 2001 |
| Grant date | Jul 6, 2004 |
| Priority date | — |
| Expiry date | May 29, 2021 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K48/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is disclosed. The fluorescence can also be monitored by observing the tissues in situ. Vertebrate subjects which contain GFP producing tumors are useful models to study the mechanism of metastasis, as well as to evaluate candidate protocols and drugs. In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.