Nucleotide sequences which code for the sahH gene

US6759224B2 · kind B2 · utility

Assignee

• Degussa AG · DE

Inventors

• Mike Farwick · Essen, DE
• Klaus Huthmacher · Gelnhausen, DE
• Jennifer Brehme · Bielefeld, DE
• Walter Pfefferle · Bodenwerder, DE
• Michael Binder · Steinhagen, DE
• Dieter Greissinger · Florstadt, DE
• Georg Thierbach · Bielefeld, DE

Key dates

• Filing date: Aug 2, 2001
• Grant date: Jul 6, 2004
• Expiry date: Jan 31, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/12
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting ofa) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2,b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2,c) polynucleotide which is complementary to the polynucleotides of a) or b), andd) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c),and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.