Mutant cell lines and methods for producing enhanced levels of recombinant proteins
US6762038B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 9, 1999 |
| Grant date | Jul 13, 2004 |
| Priority date | — |
| Expiry date | Sep 9, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/10322
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Mammalian somatic cells having a homozygous disruption in the gene which encodes the endonbonuclease RNase L and a homyzgous disruption in the gene which encodes the double-stranded RNA dependent kinase PKR are provided. Methods for producing enhanced levels of recombinant proteins in mammalian cell systems are also provided. In one aspect the method employs cells having a homozygous disruption in the RNase L gene and a homozygous disruption in the PKR gene and comprises transfecting the cells with a nucleic acid, or polynucleotide, encoding a desired, exogenous protein; expressing the exogenous protein in the cell; and isolating the exogenous protein from the transfected cells. In another aspect the method employs RNase L null cells transfected with a nucleic acid encoding a desired, exogenous protein. In another aspect the methods employ mutant cells hating a homozygous disruption in the PKR gene, i.e. PKR null cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.