Method of cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease
US6764843B2 · kind B2 · utility
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6Claims
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Key dates
| Filing date | Sep 28, 2001 |
| Grant date | Jul 20, 2004 |
| Priority date | — |
| Expiry date | Jan 11, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.