Apparatus and methods for high throughput analysis of samples in a translucent flowing liquid
US6765656B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 18, 2002 |
| Grant date | Jul 20, 2004 |
| Priority date | — |
| Expiry date | Dec 18, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6482
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Apparatus and methods for imaging multiple fluorescent sample particles in a flow channel. A flow channel defines a flow direction for samples in a flow stream and has a viewing plane perpendicular to the flow direction. A clear volume between the illuminated flow volume and the imaging optics is provided. A beam of illumination is formed as a column having a size effective to cover the viewing plane, and illuminates the flow nearly end on, or from the side. Imaging optics are arranged to view the focal plane to form an image of the multiple fluorescent sample particles in the flow stream. A camera records the image formed by the imaging optics. A computer detects and enumerates the particles in the flow.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.