Method for synthesis of nucleic acids
US6767723B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 28, 2001 |
| Grant date | Jul 27, 2004 |
| Priority date | — |
| Expiry date | Aug 15, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An object of the present invention is to provide a method of treatment and a method of storage that are useful in conducting a nucleic acid synthesis procedure capable of directly amplifying an intended nucleic acid in a living body-derived sample without purification steps.The present invention provides a method for synthesis of nucleic acids in which a living body-derived sample itself is mixed with a reaction solution for gene amplification and allowed to react, which method comprises treating the sample with a surfactant before the reaction to destruct solid components such as cells or bacterial bodies containing nucleic acids and uniformly disperse them in the sample liquid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.