Process for producing cis-3-hydroxy-L-proline
US6767726B2 · kind B2 · utility
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1References
18Claims
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Key dates
| Filing date | May 6, 2002 |
| Grant date | Jul 27, 2004 |
| Priority date | — |
| Expiry date | May 6, 2022 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/814
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.