Electron induced fluorescent method for nucleic acid sequencing
US6767731B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 27, 2001 |
| Grant date | Jul 27, 2004 |
| Priority date | — |
| Expiry date | Mar 5, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus, compositions and related methods for sequencing a target nucleic acid are described. In certain embodiments, the apparatus is a microfluidic apparatus comprising an input chamber, microchannel, output chamber and a detection unit that is operatively connected to the microchannel. In preferred embodiments, the methods include hybridizing a target nucleic acid to one or more probe libraries, moving the hybridized target nucleic acid past the detector, and detecting bound probes. Probe libraries may comprise oligonucleotides or oligonucleotide analogs, preferably with each probe uniquely labeled. A linear order of labeled probes hybridized to the target nucleic acid can be detected and the target nucleic acid sequence deduced. In preferred embodiments, probe labels are detected by analysis of electron-induced fluorescence of probes labeled with conductive polymers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.