Non-identical genes and their application in improved molecular adjuvants
US6770631B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 28, 2000 |
| Grant date | Aug 3, 2004 |
| Priority date | — |
| Expiry date | Aug 28, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/92
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides a linear concatamer of at least two non-identical DNA sequences which, by virtue of third base redundancy of the genetic code of the codons, each encode the same polypeptide of at least 30 amino acids; wherein the concatamer comprises or consists of a nucleic acid sequence which codes for an oligomer of said polypeptides in a continuous reading frame. A single invariant cysteine codon may be added to one DNA sequence to encode a polypeptide derivative with a unique unpaired cysteine. The concatamer may be fused to one or more sequences encoding one or more antigens. The DNA sequences in the concatamer may encode the compliment C3 fragment C3d or an analogue thereof. The invention also provides an expression vector comprising the concatamer nucleic acid sequence and regulatory or other sequences for expression of any oligomeric polypeptide encoded thereby, as well as a host cell comprising the expression vector. The invention can also provide a method of including a immune response to an antigen in the human or animal by administering a concatamer or administering a vector. Thus the invention can provide a pharmaceutical composition in the form of a DNA vaccin…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.