Polymer solution for separation of charged macromolecules by electrophoresis
US6770698B1 · kind B1 · utility
15Cited by
8References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 5, 2000 |
| Grant date | Aug 3, 2004 |
| Priority date | — |
| Expiry date | Sep 25, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S525/903
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is a polymer solution for the efficient separation of charged macromolecules by electrophoresis that includes a plurality of polymers. These polymers are entangled to form an interpenetrating network with greater entanglement times than corresponding homopolymers of the same length. These polymers are different and do not phase separate when dissolved in solution. Preferred polymers are PAM and PVP or PDAM and PVP. The polymer solutions of the present invention provide at least a 500-base read length in one run for a single-stranded DNA separation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.