Method for production of stroma-free hemoglobin
US6773613B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 20, 2001 |
| Grant date | Aug 10, 2004 |
| Priority date | — |
| Expiry date | Jul 20, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25375
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The invention relates to a system and a method of using an automated blood cell separator to prepare a high-quality hemoglobin solution as the raw material for manufacture of hemoglobin-based therapeutic oxygen carriers, so-called “blood substitutes”. The method of isolating stroma-free hemoglobin comprises the steps of: separating the red blood cells from the starting solution; washing the red blood cells in wash solution; contacting the red blood cells with a hypotonic solution to produce stromata and a hemolysate containing hemoglobin having an ionic strength and separating the hemolysate from the stromata. Further, the invention relates to a method for preparing a chemically modified hemoglobin solution. This method comprises the steps of: separating the red blood cells from the staring solution; washing the red blood cells in wash solution; contacting the red blood cells with a hypotonic solution to produce stromata and a hemolysate containing hemoglobin having an ionic strength; separating the hemolysate from the stromata; and mixing the hemolysate with a reagent adapted to chemically modify the hemoglobin to form a chemically modified hemoglobin solution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.