Expression vector for production of recombinant proteins

US6773919B2 · kind B2 · utility

Assignee

• Biovitrum AB · SE

Inventors

• Gunnar Norstedt · Bromma, SE
• Tim Wood · Melbourn, GB
• Daniel SLIVA · Scotia, US
• Bertil Enberg · Boo, SE
• Peter Edward Lobie · Singapore, SG
• Lars-Arne Haldosen · Örebro, SE

Key dates

• Filing date: Nov 3, 1997
• Grant date: Aug 10, 2004
• Expiry date: Nov 3, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of enhancing the transcription of a gene in a DNA construct incorporated into the genome of a eucaryotic host cell, wherein the DNA construct comprises a structural gene for a desired protein or polypeptide in a gene promoter upstream of the structural gene, comprises providing at least one enhancer element comprising the nucleotide sequence TTC TGA GAA upstream of the promoter, and exposing the DNA construct to lactogenic stimuli. An expression vector, a host cell and a transgenic mammal containing the vector are of interest.