Maize metallothionein gene and promoter
US6774282B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 7, 2000 |
| Grant date | Aug 10, 2004 |
| Priority date | — |
| Expiry date | Mar 7, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG09G2380/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a root-preferred promoter for the gene encoding, a metallothionein gene and sequences isolated therefrom. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell with a nucleotide sequence operably linked to one of the root-preferred promoters of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence in a root-preferred manner from the transformed plant cell. Compositions and methods for expressing metallothionein genes in plants, plant cells and tissues are also provided. The compositions comprise nucleotide sequences encoding plant metallothionein. The sequences are useful in transforming plants for tissue-preferred or constitutive expression of metallothionein. Such sequences find use in modulating levels of metal ions in plants and plant tissues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.