Plasmids from Corynebacterium glutamicum and use thereof

US6777229B1 · kind B1 · utility

Assignee

• Degussa AG · DE

Inventors

• Andreas Tauch · Bielefeld, DE
• Jorn Kalinowski · Bielefeld, DE
• Alfred Puhler · Bielefeld, DE
• Georg Thierbach · Bielefeld, DE

Key dates

• Filing date: Nov 3, 2000
• Grant date: Aug 17, 2004
• Expiry date: Nov 3, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P13/08
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to the mutually compatible plasmids pTET3 and pCRY4, isolated from the strain of Corynebacterium glutamicum deposited under DSM number 5616, wherein plasmid pTET3 is characterised by1.1 a length of ˜27.8 kbp and the restriction map shown in FIG. 1, 1.2 a replication region comprising the nucleotide sequence shown in SEQ ID no. 1 and1.3 an antibiotic resistance region consisting of a tetA gene imparting tetracycline resistance and an aadA gene imparting streptomycin and spectinomycin resistance, shown in SEQ ID no. 6,and plasmid pCRY4 is characterised by1.4 a length of ˜48 kbp and the restriction map shown in FIG. 2 and1.5 a replication region comprising the nucleotide sequence shown in SEQ ID no. 4to composite plasmid vectors of these plasmids which are capable of autonomous replication in coryneform bacteria and to processes for the production of L-amino acids, vitamins and nucleotides using these bacteria.