Detection and treatment of duplex polynucleotide damage
US6777405B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 3, 2002 |
| Grant date | Aug 17, 2004 |
| Priority date | — |
| Expiry date | Sep 3, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07F15/008
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Duplex polynucleotides containing damage or errors are detected with hindered intercalating compounds which are capable of intercalating only in the presence of such damage or error. Conditions characterized by the presence of polynucleotide errors or damage are treated with such compounds that are capable of catalyzing polynucleotide cleavage with light. Suitable compound include compounds of the formula:Rh(R1)(R2)(R3)3+where R1 and R2 are each independently aryl, heteroaryl, substituted aryl or substituted heteroaryl of 1 to 5 rings, and R3 is a group of the formula where x and z are each independently an integer from 1 to 4 and y is an integer from 1 to 2, and R4, R5, and R6 are each independently H—, halo, HO—, H2N—, CN—, O2N—, HS—, O3S—, O3SO—, —COOH, —CONH2, R, RO—, RNH—, RaRbN—, RO3S—, RO3SO—, —COOR, —CONHR, or —CONRaRb, where R, Ra and Rb are each independently lower alkyl, cycloalkyl, lower alkenyl, lower alkynyl, or phenyl, or two R4, R5, or R6 together form a fused aryl ring.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.