Method for mapping a DNA molecule comprising an ad infinitum amplification step
US6780593B1 · kind B1 · utility
5Cited by
3References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 18, 2002 |
| Grant date | Aug 24, 2004 |
| Priority date | — |
| Expiry date | Jan 18, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a Happy Mapping type method for mapping a DNA molecule comprising a step enabling ad infinitum amplification of the DNA of each panel, consisting of the following: (i) a first amplification with the aid of a primer comprising 10-30 nucleotides defined at extremity 5′ thereof, and 5-10 random nucleotides at extremity 3′ thereof, in addition to (ii) a second amplification with the aid of a primer comprising at least the oligonucleotides defined at extremity 5′ of the primer used in stage i.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.