Method for improving genetic stability of foreign insert nucleotide sequence in recombinant poliovirus
US6780618B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 8, 2002 |
| Grant date | Aug 24, 2004 |
| Priority date | — |
| Expiry date | Feb 8, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/32643
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to (a) methods for improving a genetic stability of an insert nucleotide sequence in a recombinant single-stranded RNA virus vector, which comprises performing a mutagenesis of the foreign insert nucleotide sequence to provide even distribution of G/C content throughout the overall foreign insert nucleotide sequence and/or to increase G/C content of the foreign insert without substantially causing amino acids substitutions (b) a recombinant single-stranded RNA virus comprising an insert nucleotide sequence with improved genetic stability and (c) a recombinant poliovirus comprising an insert nucleotide sequence with improved genetic stability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.