Ligand binding assays for vanilloid receptors
US6780650B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | Oct 11, 2001 |
| Grant date | Aug 24, 2004 |
| Priority date | — |
| Expiry date | Jan 28, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/04
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention describes an assay in which pH alters RTX affinity to VR1 receptors. The RTX affinity was increased with increasing pH from 4.1 to 8.6. Both the RTX affinity and apparent number of RTX binding sites were decreased with increasing pH from 8.6 to 10.6. The high pH may be causing the cell membranes to denature. These pH conditions (pH 8.0 to pH 9.0) provide a high signal to noise ratio, give a more robust assay and require use of less experimental materials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.