Dry analytical element for acetaminophen assay
US6783731B1 · kind B1 · utility
5Cited by
17References
8Claims
0Family size
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Key dates
| Filing date | Jun 22, 1995 |
| Grant date | Aug 31, 2004 |
| Priority date | — |
| Expiry date | Jun 22, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A spectrophotometric assay for the detection of acetaminophen in aqueous fluids is carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; and a mild oxidizing agent to oxidize the p-aminophenol so that it couples to a water-soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.