Methods for quantitative analysis of nucleic acid amplification reaction
US6783934B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 2000 |
| Grant date | Aug 31, 2004 |
| Priority date | — |
| Expiry date | May 1, 2020 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0627
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining a threshold cycle number or time value in a nucleic acid amplification reaction comprising the steps of amplifying a nucleic acid sequence in a reaction mixture; at a plurality of different times during the amplification reaction, measuring at least one signal whose intensity is related to the quantity of the nucleic acid sequence in the reaction mixture; and storing signal values defining a growth curve for the nucleic acid sequence. A derivative of the growth curve is determined and the threshold cycle number or time value is calculated as a cycle number or time value corresponding to a characteristic (e.g., maximum, minimum, or zero-crossing) of the derivative.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.