Dynamic coating with linear polymer mixture for electrophoresis
US6787016B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 2001 |
| Grant date | Sep 7, 2004 |
| Priority date | — |
| Expiry date | Apr 21, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44752
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer, where the sieving polymer is more hydrophilic than the coating polymer and is present in greater amount. Of particular interest are uncrosslinked acrylamide polymer mixtures for coating plastic channels and providing sieving for performing DNA separations in microfluidic devices. Polyacrylamide or N,N-dimethyl acrylamide is used with a N,N-dialkyl acrylamide copolymer, either separately or together for sieving and coating, serving as the medium in capillary electrophoresis DNA separations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.