Fluorogenic protease substrates based on dye-dimerization
US6787329B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 24, 1999 |
| Grant date | Sep 7, 2004 |
| Priority date | — |
| Expiry date | Nov 24, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2337/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.